@_@ tomorow=death??
Jul. 8th, 2004 12:45 pmWaahh I'm scared and nervous now. I went to get Tony (teh bossperson) to sign my timesheet and he sat me down to tell me what I'd be doing for the rest of the time I'm here. Basically, just small experments and technique learning. But that's not why I'm nervous. Tomorrow I am going to be doing another ELISA by myself. No biggie right? I did that last week...
WRONG!!!! First of all, I am doing 3 plates and each one has a different procedure to follow since they will use different cytokines. This means timing and stuff is different for all the plates and strict timekeeping will need to be kept. Second, Tony told me twice that this particular ELISA is way important so I have to be really careful to not mix stuff up. eep... no pressure... *wibbles* This is gonna be hard. I just spent the longest time staring at the 3 protocol sheets trying to figure out how I'm gonna do this and not fuck up spectacularly in the process. I mean... one of them has a full kit. The other 2 I need to make buffers and stuff. Luckily, 2 of them are similar timing, just different numbers of washes so it shouldn't be too bad. It's this 3rd that scares me. It comes in a complete kit, but the procedure is nothing like the one I did before. Yup.. I'm gonna read that one like 20 times tonight...
( Spiderman2 and not fun-ness )
WRONG!!!! First of all, I am doing 3 plates and each one has a different procedure to follow since they will use different cytokines. This means timing and stuff is different for all the plates and strict timekeeping will need to be kept. Second, Tony told me twice that this particular ELISA is way important so I have to be really careful to not mix stuff up. eep... no pressure... *wibbles* This is gonna be hard. I just spent the longest time staring at the 3 protocol sheets trying to figure out how I'm gonna do this and not fuck up spectacularly in the process. I mean... one of them has a full kit. The other 2 I need to make buffers and stuff. Luckily, 2 of them are similar timing, just different numbers of washes so it shouldn't be too bad. It's this 3rd that scares me. It comes in a complete kit, but the procedure is nothing like the one I did before. Yup.. I'm gonna read that one like 20 times tonight...
( Spiderman2 and not fun-ness )