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@_@ tomorow=death??
Waahh I'm scared and nervous now. I went to get Tony (teh bossperson) to sign my timesheet and he sat me down to tell me what I'd be doing for the rest of the time I'm here. Basically, just small experments and technique learning. But that's not why I'm nervous. Tomorrow I am going to be doing another ELISA by myself. No biggie right? I did that last week...
WRONG!!!! First of all, I am doing 3 plates and each one has a different procedure to follow since they will use different cytokines. This means timing and stuff is different for all the plates and strict timekeeping will need to be kept. Second, Tony told me twice that this particular ELISA is way important so I have to be really careful to not mix stuff up. eep... no pressure... *wibbles* This is gonna be hard. I just spent the longest time staring at the 3 protocol sheets trying to figure out how I'm gonna do this and not fuck up spectacularly in the process. I mean... one of them has a full kit. The other 2 I need to make buffers and stuff. Luckily, 2 of them are similar timing, just different numbers of washes so it shouldn't be too bad. It's this 3rd that scares me. It comes in a complete kit, but the procedure is nothing like the one I did before. Yup.. I'm gonna read that one like 20 times tonight...
In other stuff... Spiderman 2 was really good and it was fun being with the people from the lab for it. It's interesting going to that sort of movie with science peoples. For one, it leads to total dissection of the science in the movie. The prime example of this is the element that the Dr. guy uses to make the fusion reaction. Tridium is made out to be super-rare and dangerous and way cool looking. After the movie, Joe asks me, "so do you know about tridium?" He then went on to say that tridium is what they use in the lab to mark cells. It's relatively harmless, only slightly radioactive and they have vials of the stuff in the fridge in the lab. *laughs* But it was a cool movie nonetheless.
The only damper to my day was the evening when I went through an abrupt mood shift. I get home and decide to clean my room to appease my mother. In doing so, I had taken everything off my side table and was looking through it all. Doing that always unearths memories of high school and stuff which always makes me touchy. So when my sister knocked something I'd organized all over the floor I yelled at her. She left and I instantly felt rotten about it because she doesn't need me to take my anger out on her. And then I just curled up on the ground and started crying. When I finally got that out, I kept cleaning but I was still kind of upset. So then I had to get dinner for my sister and me. My mom had put a chicken in the oven and I had to cut her the wings. But I'm really bad at it because I can never hit the joint right. My dad refused to help me and I was nervous about scratching the casserole dish because my mom is spastic about that. And when it didn't work, I really just wanted to cry. Luckily I didn't, but it was a close call. *sigh* So then I was in a foul mood for the rest of the evening. It definitely wasn't cool. hmm... I think maybe I'll go back to staring at protocol sheets.....
WRONG!!!! First of all, I am doing 3 plates and each one has a different procedure to follow since they will use different cytokines. This means timing and stuff is different for all the plates and strict timekeeping will need to be kept. Second, Tony told me twice that this particular ELISA is way important so I have to be really careful to not mix stuff up. eep... no pressure... *wibbles* This is gonna be hard. I just spent the longest time staring at the 3 protocol sheets trying to figure out how I'm gonna do this and not fuck up spectacularly in the process. I mean... one of them has a full kit. The other 2 I need to make buffers and stuff. Luckily, 2 of them are similar timing, just different numbers of washes so it shouldn't be too bad. It's this 3rd that scares me. It comes in a complete kit, but the procedure is nothing like the one I did before. Yup.. I'm gonna read that one like 20 times tonight...
In other stuff... Spiderman 2 was really good and it was fun being with the people from the lab for it. It's interesting going to that sort of movie with science peoples. For one, it leads to total dissection of the science in the movie. The prime example of this is the element that the Dr. guy uses to make the fusion reaction. Tridium is made out to be super-rare and dangerous and way cool looking. After the movie, Joe asks me, "so do you know about tridium?" He then went on to say that tridium is what they use in the lab to mark cells. It's relatively harmless, only slightly radioactive and they have vials of the stuff in the fridge in the lab. *laughs* But it was a cool movie nonetheless.
The only damper to my day was the evening when I went through an abrupt mood shift. I get home and decide to clean my room to appease my mother. In doing so, I had taken everything off my side table and was looking through it all. Doing that always unearths memories of high school and stuff which always makes me touchy. So when my sister knocked something I'd organized all over the floor I yelled at her. She left and I instantly felt rotten about it because she doesn't need me to take my anger out on her. And then I just curled up on the ground and started crying. When I finally got that out, I kept cleaning but I was still kind of upset. So then I had to get dinner for my sister and me. My mom had put a chicken in the oven and I had to cut her the wings. But I'm really bad at it because I can never hit the joint right. My dad refused to help me and I was nervous about scratching the casserole dish because my mom is spastic about that. And when it didn't work, I really just wanted to cry. Luckily I didn't, but it was a close call. *sigh* So then I was in a foul mood for the rest of the evening. It definitely wasn't cool. hmm... I think maybe I'll go back to staring at protocol sheets.....
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